Project 2.7

Preliminary data indicated that a reduced phosphorus (and Ca) content in the feed influences the immune status in two hen lines. In this project, we expect that the complete removal of mineral phosphorus supplements or the addition of myo-inositol to the feed increases myo-inositol and InsP6 concentrations in the blood and that both components influence the immune system of LB and LSL hens. Important components of the gut-associated and systemic immune system are functionally characterized. The study also focusses on the expression of pro- and anti-inflammatory cytokines in lymphoid tissues as well as in target tissues that are directly related to energy metabolism and reproduction (liver, oviduct, ovary). Since pro-inflammatory cytokines also contribute to the energy and lipid metabolism, the connection between inflammation status and metabolic performance will be investigated. Given their specific immunological profile, we expect LB hens to benefit more from myo-inositol supplementation in terms of their inflammatory status. Key systems of P and Ca homeostasis (FGF23 / Klotho and vitamin D) are also directly involved in immune regulation; thus, a study of possible relationships complement the study. The immunological data from this project will also be used to analyze interrelation of various immune traits with intestinal microbiota.