Project 1.6
The influence of the microbiome and their metabolites towards epithelial functions and smooth muscle contractility in regards to InsP6 degradation
Jana Seifert / Korinna Huber / Amélia Camarinha-Silva
The overall aim of this project is to identify microbial functions in regards to InsP6 degradation and to study the capacities of the chicken epithelium for myo-inositol (MI) transport. The identification of metabolites and their producers, which stimulate the MI uptake and the gut homeostasis, will throw light on these processes of interest.
In addition, the interaction or cross-talk between the microbiota (metabolites) and the host will be investigated by a novel approach using Ussing chambers as short-term incubation systems. Possible effects towards epithelial integrity and transport functions will be described.
Smooth muscle contractility will be determined as this is the physiological basis to develop and maintain adequate gastrointestinal motility and is thereby, an essential prerequisite for proper digestion, performance and health of laying hens. Thus, simultaneous performing of two in vitro measures of tissue functions (Ussing chambers, isometric force measures) will allow to reveal a first insight in potential pathways of the cross-talk between gut microbiota and host tissues.
